Optimal Annealing Temperature Calculator What Are The Advantages And Disadvantages Of "touchdown" PCR Program?

What are the advantages and disadvantages of "touchdown" PCR program? - optimal annealing temperature calculator

Touchdown is different, then the polymerization reaction in conventional channel. The annealing temperature is the big difference in the two tenders. In conventional PCR, the temperature drops to 55 degrees C, the optimum temperature. In the note, high temperatures and low cycles until the optimum temperature. Why is this method produces better results? What sacrifices are made by the method of touchdown.